Imaging Flow Cytometry


Imaging flow cytometry is a powerful technique for high-throughput single-cell analysis. It provides comprehensive analysis and in-depth imagery of every individual cell and is used in applications such as cancer screening.

Blood cells are aligned in a microfluidic channel and scanned by an ultrafast pulsed laser beam, and the output is captured by high-performance digitizers via photomultiplier tubes (PMTs). Both the flow speed and image quality are superior to conventional CMOS-based approaches, and due to the high throughput single-cell analysis and characterization enable high statistical accuracy by millions of captured cell images.

What to consider when selecting a digitizer for flow cytometry
  • High dynamic range is required to achieve superior image quality
  • High sampling rate enables high microfluidic channel flow rate and system-level throughput
  • Open FPGA is crucial for real-time image pre-processing
  • High data transfer rate is needed in order to support high-speed image post-processing and data storage
  • Peer-to-peer GPU streaming offers additional benefits for cell characterization
Commonly Used Products and Features

Our imaging flow cytometry customers typically use ADQ7DC in PCIe form factor. It is a single-/dual-channel, 14-bit digitizer with up to 10 GSPS sampling rate and it features a large on-board Xilinx Kintex Ultrascale FPGA which is open to the user.

Furthermore, ADQ7DC supports GPU peer-to-peer streaming at rates up to 6.8 GByte/s across a PCIe Gen3 x8 bus. Optional firmware and development kits can be purchased separately at any time and upgrades can be done in place without any need for system disassembly or significant downtime.

Further Reading / Next Steps
  • See how University of Hong Kong (HKU) achieved line scan rates of 10M lines/s using ADQ7DC here
  • For a list of key benefits and specifications please visit the ADQ7DC product page here
  • For information about the optional firmware packages and development kit see here
  • "The technical and intellectual support from the team at Teledyne SP Devices has been playing an important role in our research."

    Associate Professor at Hong Kong University (HKU) who has implemented a system supporting line scan rates of 10M lines/s.

  • Flow Cytometry
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  • Flow Cytometry
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  • Flow Cytometry
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  • Flow Cytometry
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    • Single/dual channel
    • Up to 10 GSPS sampling rate
    • Up to 3 GHz input bandwidth
    • DC-coupled

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